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KMID : 0368420020450010056
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Journal of Plant Biology
2002 Volume.45 No. 1 p.56 ~ p.61
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Plant Regeneration and Morphology during in-Vitro Organogenesis from Heloniopsis orientalis(Thunb.) C. Tanaka
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Cha, Hyeon-Cheol
Park, Hye-Jeong/Min, Byeong-Mee
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Abstract
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Heloniopsis orientalis (Liliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. Leaf tissues were cultured on MS basal media supplemented with growth regulators 2,4-D, TDZ, BA, or zeatin. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.1 to 1.0㎎/L 2,4-D or 0.1 to 3.0㎎/L BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 0.1㎎/L BA. TDZ triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. After the plants were acclimatized for one month at 4℃, they were successfully transferred to soil. In addition, we used LM and SEM to investigate shoot morphogenesis at various stages of differentiation.
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